First, T-2 toxin reacted with succinic anhydride in steam-bath to synthesize T-2 hemisuccinate ( T-2HS). Thin layer chromatography analysis showed that the objective hapten was obtained successfully. 在蒸气浴条件下,T-2毒素与琥珀酸酐(HS)反应合成了T-2HS,薄层层析显示,目标半抗原合成成功;
The result showed that T-2 toxin could conjugate with BSA or OVA to produce T-2 toxin antigen. 结果显示,T-2HS能与牛血清白蛋白(BSA)和卵清蛋白(OVA)结合生成T-2毒素抗原。
The results indicated that T-2 toxin had an effect on ATP metabolism, and the major organ involved in disturbance of energy metabolism was the chicken liver and Selenium played a significant role in preventing the decrease in ATP concentration caused by T-2 toxin. 硒对T-2毒素引起的肝脏ATP减少有保护作用。
Conclusion The obtained polyclonal anti-idiotype antibody was the "inner image" with T-2 toxin and can be used to replace T-2 toxin standard in ELISA. 结果所研制的抗独特型抗体经过鉴定,是Ab2β型,与T-2毒素存在内影像关系。结论该抗体可以替代T-2毒素标准品应用于ELISA检测。
Conclusion A monoclonal antibody against T-2 toxin was prepared, which makes a solid foundation for establishment of immunological methods for detecting T-2 toxin levels in food, feed and biological fluid. 结论成功制备出抗T-2毒素单克隆抗体,为建立测定食品、饲料和组织液中T-2毒素的免疫学方法打下了坚实的基础。
Conclusion T-2 toxin can obviously inhibit aggrecan and collagen ⅱ synthesis in human chondrocytes, and selenium can partly antagonize the inhibitory effects of T-2 toxin on aggrecan. 结论T-2毒素对软骨细胞Ⅱ型胶原蛋白和蛋白聚糖合成有明显的抑制作用,微量元素硒对T-2毒素引起的软骨细胞蛋白聚糖改变有一定保护作用。
In order to find out whether rhG-CSF is effective to WBC decrease disease induced by food toxin, WBC decrease disease models induced by T-2 toxin. 为探讨rhG-CSF对食物中毒致模型动物白细胞(WBC)减少症的有效性,以T-2毒素中毒致WBC减少症模型进行了研究。
Conclusion T-2 toxin of minidoses can cause many biochemical indexes change, these results show that T-2 toxin of minidoses can cause damage in many organs of rat. 结论低剂量T鄄2毒素可致机体多种生化指标改变,提示低剂量T鄄2毒素可能引起机体多器官损伤。
Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA, then BSA T 2HS was prepared. 方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应,生成T-2-半琥珀酸酐后,再与小牛血清白蛋白经碳二亚胺偶联结合生成T-2毒素抗原。
The overexpression of Bcl-2 and Bax were detected and Bax/ Bcl-2 ratio was increased after T-2 toxin treated chondrocytes. Selenium could partly block the apoptosis of chondrocytes induced by T-2 toxin. 硒能部分对抗T2毒素引起的Bcl2和Bax表达的提高,使Bax/Bcl2比值下降,以降低凋亡率。
Polyclonal antibody against T-2 toxin was produced using T-2 toxin immunity rabbits. It could be used to determine T-2 toxin in corn. 用T-2毒素免疫家兔,获得了抗T-2毒素的多克隆抗体,运用该抗体建立了检测玉米中T-2毒素的间接竞争性酶联免疫吸附测定法。
Objective To investigate the damage of organism induced by mini-dose T-2 toxin, and to lay a theory foundation for constituting the standard of T-2 toxin in cereals in the future. 目的观察低剂量T-2毒素对机体的损伤作用,为将来制定粮食中T-2毒素含量卫生标准奠定理论基础。